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CRL-1648 CA46 Burkitts 人淋巴瘤细胞系，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和最优培养条件！
CRL-1648 CA46 Burkitts 人淋巴瘤细胞系 的详细介绍
CRL-1648 CA46 Burkitts 人淋巴瘤细胞系
ATCC® Number:
CRL-1648™
Price:
$399.00
Designations:
CA46
Depositors:
IT Magrath
Isotype:
IgM; kappa light chain
Biosafety Level:
1
Shipped:
frozen
Medium & Serum:
See Propagation
Growth Properties:
suspension
Organism:
Homo sapiens (human)
Morphology:
lymphoblast
Source:
Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Cellular Products:
immunoglobulin (surface and secreted)
Permits/Forms:
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:
complement
Antigen
HLA B27 (the patient was HLA A2, A11, B17, B27)
DNA Profile (STR):
Amelogenin: X
CSF1PO: 10,12
D13S317: 8,12
D16S539: 11,12
D5S818: 13
D7S820: 11,12
THO1: 7,9
TPOX: 8,9
vWA: 15,16
Cytogenetic Analysis:
46, XO, XY, dup1(q21-32), t(8;14) (8pter --> 8q23 :: 14q32 --> 14qter; 14pter --> 14q32 :: 8q23 --> 8qter), 13q+, +16
Gender:
male
Comments:
The cells are EBNA negative and are negative for Fc receptors.
Approximately 12% of the cells express commplement receptors.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml.
A saturation density of 2 X 10 exp6 is obtainable.
Preservation:
culture medium 95%; DMSO, 5%