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CRL-7601 Hs 865.Sk 人皮肤成纤维细胞，ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|。细胞库管理规范，提供的细胞株背景清楚，提供参考文献和最优培养条件!
CRL-7601 Hs 865.Sk 人皮肤成纤维细胞 的详细介绍
CRL-7601 Hs 865.Sk 人皮肤成纤维细胞
ATCC® Number:
CRL-7601™
Price:
Designations:
Hs 865.Sk
Biosafety Level:
1
Shipped:
frozen
Medium & Serum:
See Propagation
Growth Properties:
adherent
Organism:
Homo sapiens (human)
Morphology:
fibroblast
Source:
Organ: skin
Disease: normal
Permits/Forms:
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age:
59 years
Gender:
female
Ethnicity:
Caucasian
Comments:
The line was established from apparently normal tissue from a patient who had adenocarcinoma of the stomach.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository de
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation:
culture medium 95%; DMSO, 5%
Related Products:
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020